A0480
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 | Immunofluorescence Arabidoposis suspension cells labeled with the Monoclonal Anti-Actin, clone 10-B3 (MAbGPa) (Cat. No. A0480) at a 1:100 dilution. The actin filaments were revealed with Anti-Mouse IgG-FITC (Cat. No. F6257) (green) and the nucleus is stained with DAPI (red). From M.K. Kandasamy, Genetics Department, University of Georgia, Athens, GA. |
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A0480
Sigma
Monoclonal Anti-Actin (plant) antibody produced in mouse
clone 10-B3 (MAbGPa), purified immunoglobulin, buffered aqueous solution
| MDL number: | MFCD00145889 |
Description
| Immunogen | ACT8 protein. |
| Specificity | The antibody recognizes actin in many plant species and in Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12). |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | 10-B3 (MAbGPa) (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~45 kDa |
| species reactivity | plant |
| application(s) | immunoblotting: 1-2 μg/mL using corn leaves extracts |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| isotype | IgG2b |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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References
| reference | De la Canal, L., et al., Phospholipids are present in extracellular fluids of imbibing sunflower seeds and are modulated by hormonal treatments. J. Exp. Bot. 59, 553-562, (2008) Abstract |
